NR4A1 antibody (AA 324-373)

Catalog No. ABIN6748603

The Rabbit Polyclonal anti-NR4A1 antibody (ABIN6748603) specifically detects NR4A1 in WB, IHC and IHC (p). The antibody is reactive with Human, Dog, Cow, Horse, Rabbit and Guinea Pig samples.

Quick Overview for NR4A1 antibody (AA 324-373) (ABIN6748603)

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Reactivity: Human, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR4A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NR4A1 Antibody

Binding Specificity: AA 324-373

Specificity: Human NR4A1 / NUR77

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Rabbit, Guinea pig (100%) Mouse, Rat (92%) Bovine (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa324-373 of human NR4A1 (P22736, NP_002126). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Elephant, Panda, Dog, Rabbit, Horse, Guinea pig, Zebra finch (100%), Marmoset, Mouse, Rat, Bat (92%), Bovine (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS buffer with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NR4A1

Target: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Alternative Name: NR4A1 / NUR77

Background: Name/Gene ID: NR4A1
Subfamily: NR4 Nerve growth factor-like
Family: NHR

Synonyms: NR4A1, GFRP1, Early response protein NAK1, HMR, NGFIB, Orphan nuclear receptor TR3, Orphan receptor tr3, Steroid receptor TR3, TR3, NAK1, NGFI-B, NP10, Receptor NGFIB, Testicular receptor 3, TR3 orphan receptor, N10, NAK-1, NUR77, Orphan nuclear receptor HMR

Gene ID: 3164

NCBI Accession: NP_002126

UniProt: P22736

Pathways: Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Steroid Hormone Mediated Signaling Pathway