Eukaryotic Translation Initiation Factor 2, Subunit 3 Gamma, 52kDa (EIF2S3) antibody

Antigen: Eukaryotic Translation Initiation Factor 2, Subunit 3 Gamma, 52kDa (EIF2S3)

Synonyms: fi03g03, zgc:63805, Su(var)3-9, wu:fi03g03, wu:fi37d04, eif2s3, MGC53362, EIF2S3, EIF2, EIF2G, eIF-2gA, EIF2gamma, eif2, eif2g, eif2s3y, eif2gamma, MGC137622, DKFZp469N0112

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Chicken, Dog (Canine), Xenopus laevis

Application: Western Blotting (WB)

Catalog no.: ABIN502695

Additional Information

Alternative name: EIF2G / EIF2S3

Gene ID: EIF2S3

UniProt: P41091

Immunogen: The immunogen for anti-EIF2S3 antibody: synthetic peptide directed towards the N terminal of human EIF2S3

Sequence: AGGEAGVTLGQPHLSRQDLTTLDVTKLTPLSHEVISRQAT INIGTIGHVA

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-EIF2S3 antibody concentration is 1 mg/ml.

Description: EIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Characteristics: Antigen Size: 472 amino acids

Molecular Weight: 51kDa

Application Details

Application Notes: WB Suggested Anti-EIF2S3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Transfected 293T.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-EIF2S3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Transfected 293T

Publications

Product: Tu, Yan, Hood et al.: "Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 4, pp. 575-88, 2007 (PubMed).