CAD antibody (N-Term)

Catalog No. ABIN502703

The Rabbit Polyclonal anti-CAD antibody is suitable to detect CAD in samples from Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea Pig, Cow, Zebrafish (Danio rerio) and Sheep. It has been validated for WB.

Quick Overview for CAD antibody (N-Term) (ABIN502703)

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea Pig, Cow, Zebrafish (Danio rerio), Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CAD Antibody

Binding Specificity: N-Term

Sequence: AALVLEDGSV LRGQPFGAAV STAGEVVFQT GMVGYPEALT DPSYKAQILV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 86%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against CAD. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CAD

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 2225 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CAD

Target: CAD (Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, and Dihydroorotase (CAD))

Alternative Name: CAD

Background: CAD is a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: -
Protein Interaction Partner: UBC, TUBG1, TP53, SUMO2, SPRTN, STAU1, LGR4, SUMO1, NEDD8, WWOX, NEDD4, FBXO6, HDAC6, ILK, CDK2, HDAC11, HTRA2, TP63, NOS2, ITGA4, RUVBL1, XPO1, VARS, TUBB2A, TUBA4A, SMARCC2, SMARCA4, RPS3, PSMD1, PSMC2, RUVBL2, TUBB4B, SHC1, SMAD2, GRB2, CBL, CUL3, FBXO
Protein Size: 2225

Molecular Weight: 243 kDa

Gene ID: 790

NCBI Accession: NM_004341, NP_004332

UniProt: P27708

Pathways: Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process