Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (N-Term) antibody

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Antigen
Synonyms FLH21957, HGK, MEKKK4, NIK, 9430080K19Rik, AU043147, AU045934, AW046177, Nik
Epitope
N-Term
(31), (21), (17), (15), (15), (10), (10), (7), (7), (6), (6), (6), (6), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(153), (45), (24), (1), (1), (1), (1)
Host
Rabbit
(131), (30)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (8), (8), (7), (7), (7), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(95), (86), (36), (22), (16), (16), (14), (4), (4), (3), (2), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human MAP4K4
Blocking Peptide Blocking peptide for this product available: Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (N-Term) peptide (ABIN979786)
Sequence PFIRDQPNER QVRIQLKDHI DRTRKKRGEK DETEYEYSGS EEEEEEVPEQ
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against MAP4K4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MAP4K4 (MAP4K4 Antibody Abstract)
Background MAP4K4 is the serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. MAP4K4 appears to act upstream of the JUN N-terminal pathway.The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Molecular Weight 133 kDa
Gene ID 9448
NCBI Accession NM_004834, NP_004825
UniProt O95819
Research Area Signaling
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1166 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (N-Term) antibody (ABIN503011) WB Suggested Anti-MAP4K4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (N-Term) antibody (ABIN503011) anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (N-Term) antibody (Image 2)
Background publications Bouzakri, Zierath: "MAP4K4 gene silencing in human skeletal muscle prevents tumor necrosis factor-alpha-induced insulin resistance." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 7783-9, 2007 (PubMed).

Catalog No. ABIN503011
$289.00
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