Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) antibody
| Antigen |
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| Synonyms |
HGK, NIK, FLH21957, FLJ10410, FLJ20373, FLJ90111, KIAA0687 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Chicken, Zebrafish, Pig (Porcine)
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Conjugate
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Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Application
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Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
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| Catalog no. |
ABIN503011 |
| Quantity |
50 µg |
| Price |
317.90 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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MAP4K4
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Gene ID
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MAP4K4
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Immunogen
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The immunogen for anti-MAP4K4 antibody: synthetic peptide directed towards the N terminal of human MAP4K4
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Sequence
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PFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGS EEEEEEVPEQ
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Cross-Reactivity
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Cow (Bovine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
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Format
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Lyophilized
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Reconstitution
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Add 50 µl of distilled water. Final anti-MAP4K4 antibody concentration is 1 mg/ml.
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Description
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MAP4K4 is the serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. MAP4K4 appears to act upstream of the JUN N-terminal pathway.The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
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Characteristics
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Antigen Size: 1166 amino acids
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Molecular Weight
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133kDa
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Application Notes
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WB Suggested Anti-MAP4K4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Small Intestine.
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Purification
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Affinity Purified
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Buffer
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PBS
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Storage (Long Term)
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For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Storage (Short Term)
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Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
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Research Area
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Signaling
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Restrictions
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For Research Use only
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Product
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Bouzakri, Zierath: "MAP4K4 gene silencing in human skeletal muscle prevents tumor necrosis factor-alpha-induced insulin resistance." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 7783-9, 2007 (PubMed).
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Alternatives for antigen "Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4)", type "Antibodies"
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Hosts
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Rabbit (59), Mouse (12)
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Reactivities
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Human (69), Mouse (Murine) (37), Chicken (19), Cow (Bovine) (19), Dog (Canine) (19), Horse (Equine) (19), Pig (Porcine) (19), Rat (Rattus) (19)
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Applications
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Immunofluorescence (IF) (35), ELISA (28), Western Blotting (WB) (28), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
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Epitopes
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pSer631 (7), pSer629 (6), pSer801 (6), AA 400-500 (3), AA 931-947 (2), C-Term (1), Center (1), Internal Region (1), N-Term (1), pSer63 (1), pTyr1238 (1)
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2013 BioMed Central Research Awards
