SET and MYND Domain Containing 2 (SMYD2) (Middle Region) antibody

Details for Product No. ABIN503176
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Antigen
Synonyms HSKM-B, KMT3C, ZMYND14, 1110020E07Rik, 4930402C15, Zmynd14, smyd2-a, im:7146196, smyd2, wu:fc02f02, zgc:110553
Epitope
Middle Region
(7), (7), (5), (2), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Pig (Porcine), Dog (Canine), Cow (Bovine), Zebrafish (Danio rerio), Human, Chicken
(49), (27), (17), (13), (13), (13), (1), (1)
Host
Rabbit
(44), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)
(35), (17), (10), (10), (4), (4), (3), (1), (1)
Pubmed 2 references available
Quantity 50 µg
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Catalog No. ABIN503176
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human SMYD2
Sequence SMWLKLGRLY MGLEHKAAGE KALKKAIAIM EVAHGKDHPY ISEIKQEIES
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Chicken : 90 %, Guinea pig : 90 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against SMYD2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SMYD2
Background The specific function of this protein remains unknown.SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).
Molecular Weight 50 kDa
Gene ID 56950
NCBI Accession NP_064582, NM_020197
UniProt Q9NRG4
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 433 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-SET and MYND Domain Containing 2 (SMYD2) (Middle Region) antibody WB Suggested Anti-SMYD2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: SH-SYSY cell lysate
anti-SET and MYND Domain Containing 2 (SMYD2) (Middle Region) antibody (2) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Product cited in: Shibamoto, Ogino, Suzuki et al.: "Primary central nervous system lymphoma in Japan: changes in clinical features, treatment, and prognosis during 1985-2004." in: Neuro-oncology, Vol. 10, Issue 4, pp. 560-8, 2008 (PubMed).

Background publications Abu-Farha, Lambert, Al-Madhoun et al.: "The tale of two domains: proteomics and genomics analysis of SMYD2, a new histone methyltransferase." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 560-72, 2008 (PubMed).

Validation Images
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