PRKACB antibody (Middle Region)

Catalog No. ABIN503961

The Rabbit Polyclonal anti-PRKACB antibody (ABIN503961) specifically detects PRKACB in WB. The antibody is reactive with Human, Rat, Mouse, Cow, Guinea Pig, Horse, Rabbit, Dog, Sheep and Zebrafish (Danio rerio) samples.

Quick Overview for PRKACB antibody (Middle Region) (ABIN503961)

Target: PRKACB (Protein Kinase, CAMP Dependent, Catalytic, beta (PRKACB))

Reactivity: Human, Rat, Mouse, Cow, Guinea Pig, Horse, Rabbit, Dog, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKACB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRKACB Antibody

Binding Specificity: Middle Region

Sequence: NGVSDIKTHK WFATTDWIAI YQRKVEAPFI PKFRGSGDTS NFDDYEEEDI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 92%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against PRKACB. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PRKACB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 351 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRKACB

Target: PRKACB (Protein Kinase, CAMP Dependent, Catalytic, beta (PRKACB))

Alternative Name: PRKACB

Background: CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase.
Alias Symbols: DKFZp781I2452, MGC41879, MGC9320, PKACB
Protein Interaction Partner: APPBP2, UBC, TP53RK, TPRKB, KIAA1279, ARPC2, ACTR2, ACTR3, ARPC1B, ARPC4, SUMO1, PFDN1, CDK5RAP2, MAPRE1, AKAP11, DCAF7, AKAP9, TRAP1, PDE4DIP, AKAP5, AKAP7, VAPA, VAPB, DYNLL1, AKAP1, RPS6, RPL27A, PRKAR2B, PRKAR2A, PRKAR1B, PRKAR1A, PRKACA, HSP90AB1, HS
Protein Size: 351

Molecular Weight: 40 kDa

Gene ID: 5567

NCBI Accession: NM_002731, NP_002722

UniProt: P22694

Pathways: AMPK Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Lipid Metabolism