Centromere Protein H (CENPH) antibody

Antigen: Centromere Protein H (CENPH)

Synonyms: ENP, CENP-H, AU044255, 1700021I11Rik, 2410018A12Rik, 2610042E16Rik, 2810046K12Rik, MGC127326, si:dkey-186m8.3, cenp-h, NNF1, PMF1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Dog (Canine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN504443

Additional Information

Alternative name: CENP-H

Gene ID: CENPH

UniProt: Q9H3R5

Immunogen: The immunogen for anti-CENPH antibody: synthetic peptide directed towards the middle region of human CENPH

Sequence: ESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKID LDSMENSERI

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-CENPH antibody concentration is 1 mg/ml.

Description: Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. CENPH colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Characteristics: Antigen Size: 247 amino acids

Molecular Weight: 27kDa

Application Details

Application Notes: WB Suggested Anti-CENPH Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Liver.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-CENPH Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Liver

Publications

Product: Liao, Zhang, Zhou et al.: "Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity." in: Cancer research, Vol. 67, Issue 17, pp. 8131-8, 2007 (PubMed).