ATP6V1E2 antibody (Middle Region)

Catalog No. ABIN504500

The Rabbit Polyclonal anti-ATP6V1E2 antibody is suitable to detect ATP6V1E2 in samples from Human, Mouse, Rat, Cow, Dog, Sheep, Guinea Pig, Horse and Rabbit. It has been validated for WB.

Quick Overview for ATP6V1E2 antibody (Middle Region) (ABIN504500)

Target: ATP6V1E2 (ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E2 (ATP6V1E2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1E2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6V1E2 Antibody

Binding Specificity: Middle Region

Purpose: ATP6V1E2 Antibody - middle region

Sequence: LMSTMRNQAR LKVLRARNDL ISDLLSEAKL RLSRIVEDPE VYQGLLDKLV

Predicted Reactivity: Cow: 79%, Dog: 79%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 79%, Rat: 86%, Sheep: 79%

Characteristics: This is a rabbit polyclonal antibody against ATP6V1E2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATP6V1E2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58590-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATP6V1E2

Target: ATP6V1E2 (ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E2 (ATP6V1E2))

Alternative Name: ATP6V1E2

Background:

Targeted Subunit: E 2

Background Information: ATP6V1E2 is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome.

Gene Name: ATPase, H+ transporting, lysosomal 31 kDa, V1 subunit E2

Alternative Symbols: VMA4, ATP6E1, ATP6EL2, ATP6V1EL2

Protein Name: V-type proton ATPase subunit E 2

Molecular Weight: 26kDa

Gene ID: 90423

NCBI Accession: NP_542384

UniProt: Q96A05

Pathways: Transition Metal Ion Homeostasis, Proton Transport