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ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E2 (ATP6V1E2) (Middle Region) antibody

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Synonyms ATP6E1, ATP6EL2, ATP6V1EL2, VMA4, 4930500C14Rik, Atp6e1, E1
Middle Region
(6), (3), (2), (2), (1)
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(17), (5), (4)
(14), (4)
Western Blotting (WB)
(14), (5), (3), (1)
Pubmed 1 reference available
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Quantity 50 μg
Shipping to United States ( )
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Immunogen Synthetic peptide directed towards the middle region of human ATP6V1E2
Blocking Peptide Blocking peptide for this product available: ABIN973636
Predicted Reactivity Asian toad :85 %,   American bullfrog :85 %,   Chicken :85 %,   Rainbow smelt :85 %,   Rat :75 %,   Sheep :75 %,   Crab-eating macaque :75 %,   Mouse :75 %,   Bovine :75 %,   Dog :75 %,   Leschenault's rousette :75 %,   Indian short-nosed fruit bat :75 %,   Schreibers's long-fingered bat :75 %,   Lesser asiatic yellow bat :75 %,   Zebra finch :75 %,   Human :105 %,   Atlantic salmon :75 %,   Sablefish :75 %,   Green puffer :75 %,  
Characteristics This is a rabbit polyclonal antibody against ATP6V1E2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name ATP6V1E2 (ATP6V1E2 Antibody Abstract)
Background ATP6V1E2 is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome.
Molecular Weight 26 kDa
Gene ID 90423
NCBI Accession NP_542384, NM_080653
UniProt Q96A05
Research Area Signaling, Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.

Antigen size: 226 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E2 (ATP6V1E2) (Middle Region) antibody (ABIN504500) WB Suggested Anti-ATP6V1E2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Background publications Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Catalog No. ABIN504500
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