Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) (Middle Region) antibody

Details for Product No. ABIN504581
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Antigen
Synonyms RIPK2, 2210420D18Rik, CARD3, CARDIAK, CCK, D4Bwg0615e, RICK, RIP2, GIG30
Epitope
Middle Region
(20), (15), (13), (6), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Human, Chicken
(110), (39), (20), (1), (1), (1)
Host
Rabbit
(92), (13), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1)
Application
Immunofluorescence (IF), Western Blotting (WB)
(107), (60), (35), (27), (12), (8), (2), (2), (2)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN504581
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human RIPK2
Sequence LRQERKRPLL DLHIELNGYM YDWNSRVSAK EKYYVWLQHT LRKKLILSYT
Predicted Reactivity Bovine : 85 %, Dog : 85 %, Horse : 85 %, Human : 85 %, Rabbit : 85 %, Chicken : 75 %, Guinea pig : 75 %, Mouse : 75 %, Rat : 75 %
Characteristics This is a rabbit polyclonal antibody against RIPK2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name RIPK2
Background RIPK2 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. It contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase activation and recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.
Molecular Weight 61 kDa
Gene ID 8767
NCBI Accession NM_003821, NP_003812
UniProt O43353
Research Area Apoptosis/Necrosis, Signaling
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 540 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) (Middle Region) antibody WB Suggested Anti-RIPK2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate
anti-Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) (Middle Region) antibody (2) Sample Type: Rat thyrocytes-FRTL-5

Primary Antibody Dilution: 1:100

Secondary Antibody: Anti-rabbit-Texas Red

Secondary Antibody Dilution: 1:000
Color/Signal Descriptions: Red: RIPK2Blue: DAPI

Gene Name: RIPK2


Submitted by: Syed A Morshed, Mount Sinai School of Medicine and James J Peters VA Medical Center
Background publications Hasegawa, Fujimoto, Lucas et al.: "A critical role of RICK/RIP2 polyubiquitination in Nod-induced NF-kappaB activation." in: The EMBO journal, Vol. 27, Issue 2, pp. 373-83, 2008 (PubMed).

Validation Images
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