V5 Epitope Tag antibody

Catalog No. ABIN559686

The Mouse Monoclonal anti-V5 Epitope Tag antibody (Clone E10) (ABIN559686) specifically detects V5 Epitope Tag in WB, ELISA, IP, DB and ISt.

Quick Overview for V5 Epitope Tag antibody (ABIN559686)

Target: V5 Epitope Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This V5 Epitope Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunostaining (ISt)

Clone: E10

Product Details anti-V5 Epitope Tag Antibody

Sequence: GKPIPNPLLG LDST

Specificity: Recognizes V5-tagged fusion proteins

Sensitivity: WB (with ECL): 1-2ng of V5-tagged fusion proteins

Purification: Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor

Immunogen: GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH

Isotype: IgG1

Application Details

Application Notes: WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Restrictions: For Research Use only

Handling

Concentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for V5 Epitope Tag

Target: V5 Epitope Tag

Alternative Name: V5-Tag

Target Type: Tag