DYKDDDDK (Flag) Tag antibody
| Antigen |
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| Clonality |
Monoclonal (FG4R) |
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Host
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Application
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Alternatives Dot Blot (Dot), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Western Blotting (WB)
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| Catalog no. |
ABIN559688 |
| Quantity |
100ug (1mg/mL) |
| Price |
179.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Immunogen
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DYKDDDDK (FLAG) synthetic peptide conjugated to KLH
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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FG4R
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Specificity
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Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
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Sensitivity
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WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins
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Application Notes
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WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
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Concentration
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1mg/mL
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Purification
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Protein A affinity chromatography from mouse ascites fluid
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Buffer
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10mM PBS, pH 7.2, 0.05% NaN3
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Storage
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Store at -20°C for up to 2 years or more. Centrifuge after first thaw to maximize product recovery. Avoid repeated freeze-thaw cycles by aliquots. Store aliquots at 4°C for several days to weeks
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Restrictions
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For Research Use only
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Alternatives for antigen "DYKDDDDK (Flag) Tag", type "Antibodies"