ATP5E antibody (AA 1-51)

Catalog No. ABIN560001

Cited in 4+ publications. The Mouse Monoclonal anti-ATP5E antibody (Clone 2F3) (ABIN560001) specifically detects ATP5E in ELISA, WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for ATP5E antibody (AA 1-51) (ABIN560001)

Target: ATP5E (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, epsilon Subunit (ATP5E))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATP5E antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2F3

Product Details anti-ATP5E Antibody

Binding Specificity: AA 1-51

Purpose: Mouse monoclonal antibody raised against a full length recombinant ATP5E.

Sequence: MVAYWRQAGL SYIRYSQICA KAVRDALKTE FKANAEKTSG SNVKIVKVKK E

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: ATP5E (AAH01690, 1 a.a. ~ 51 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-ATP5E antibody (ABIN560001)

Fujikawa, Sugawara, Tanabe, Yoshida: "Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex." in: FEBS letters, Vol. 589, Issue 19 Pt B, pp. 2707-12, (2015) (PubMed).

Fujikawa, Imamura, Nakamura, Yoshida: "Assessing actual contribution of IF1, inhibitor of mitochondrial FoF1, to ATP homeostasis, cell growth, mitochondrial morphology, and cell viability." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18781-7, (2012) (PubMed).

Havlícková, Kaplanová, N?sková, Drahota, Houstek: "Knockdown of F1 epsilon subunit decreases mitochondrial content of ATP synthase and leads to accumulation of subunit c." in: Biochimica et biophysica acta, Vol. 1797, Issue 6-7, pp. 1124-9, (2010) (PubMed).

Mayr, Havlícková, Zimmermann, Magler, Kaplanová, Jesina, Pecinová, Nusková, Koch, Sperl, Houstek: "Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 epsilon subunit." in: Human molecular genetics, Vol. 19, Issue 17, pp. 3430-9, (2010) (PubMed).

Target Details for ATP5E

Target: ATP5E (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, epsilon Subunit (ATP5E))

Alternative Name: ATP5E

Background: Full Gene Name: ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit
Synonyms: ATPE,MGC104243

Gene ID: 514

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process