ATP5E antibody (Internal Region)

Catalog No. ABIN6260125

Product Details anti-ATP5E Antibody

Target: ATP5E (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, epsilon Subunit (ATP5E))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5E antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: ATP5E Antibody detects endogenous levels of total ATP5E.

Predicted Reactivity: Pig,Bovine

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATP5E, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATP5E

Target: ATP5E (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, epsilon Subunit (ATP5E))

Alternative Name: ATP5E (ATP5E Products)

Synonyms: 2410043G19Rik antibody, ATPE antibody, AV000645 antibody, MC5DN3 antibody, DDBDRAFT_0217763 antibody, DDBDRAFT_0233319 antibody, DDB_0217763 antibody, DDB_0233319 antibody, ATP5E antibody, ATPsynepsilon antibody, BcDNA:RE19513 antibody, BcDNA:RH48911 antibody, CG9032 antibody, Dmel\CG9032 antibody, Sun antibody, l(1)13Fe antibody, l(1)XV antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit antibody, ATP synthase epsilon chain, mitochondrial antibody, mitochondrial F1F0 ATP synthase epsilon subunit antibody, ATP synthase epsilon chain antibody, stunted antibody, Atp5e antibody, ATP5E antibody, atp5e antibody, LOC100347099 antibody, meatpase antibody, atpE antibody, sun antibody

Background:

Description: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits (By similarity).

Gene: ATP5E

Molecular Weight: 6 kDa

Gene ID: 514

UniProt: P56381

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process