Interferon Regulatory Factor 1 (IRF1) (AA 216-325) antibody

Antigen: Interferon Regulatory Factor 1 (IRF1)

Synonyms: MAR, IRF-1, Irf-1, AU020929, MGC136945, zgc:136945, IRF1, MGC103566, MGC192360, zgc:103566

Binding Site: AA 216-325

Clonality: Monoclonal (2E4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN561520

Additional Information

Alternative name: IRF1

Gene ID: 3659

NCBI Acession: NM_002198, NP_002189

Immunogen: IRF1 (NP_002189, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: PMPSTSEATTDEDEEGKLPE DIMKLLEQSEWQPTNVDGKG YLLNEPGVQPTSVYGDFSCK EEPEIDSPGGDIGLSLQRVF TDLKNMDATWLDSLLTPVRL PSIQAIPCAP

Cross-Reactivity: Human

Isotype: IgG2a kappa

Clone: 2E4

Description: Gene: interferon regulatory factor 1
Synonyms: IRF-1, MAR

Characteristics: Mouse monoclonal antibody raised against a partial recombinant IRF1.

Application Details

Application Notes: Application specialization: Cell lysate, Recombinant protein

Buffer: 1x PBS, pH 7.2

Storage: -20 °C

Storage Comment: Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

IRF1 monoclonal antibody (M01), clone 2E4 Western Blot analysis of IRF1 expression in COLO 320 HSR

Detection limit for recombinant GST tagged IRF1 is approximately 0.1ng/ml as a capture antibody.

Western Blot detection against Immunogen (37.84 KDa) .

Publications

Product: Ye, Lista, Lo Coco et al.: "Alterations of a zinc finger-encoding gene, BCL-6, in diffuse large-cell lymphoma." in: Science (New York, N.Y.), Vol. 262, Issue 5134, pp. 747-50, 1993 (PubMed).

Steffen, MacKay, Kerr et al.: "Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4." in: Cell, Vol. 133, Issue 2, pp. 292-302, 2008 (PubMed).

Fazilleau, McHeyzer-Williams, Rosen et al.: "The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding." in: Nature immunology, Vol. 10, Issue 4, pp. 375-84, 2009 (PubMed).

Nurieva, Chung, Martinez et al.: "Bcl6 mediates the development of T follicular helper cells." in: Science (New York, N.Y.), Vol. 325, Issue 5943, pp. 1001-5, 2009 (PubMed).

Choi, Kageyama, Eto et al.: "ICOS receptor instructs T follicular helper cell versus effector cell differentiation via induction of the transcriptional repressor Bcl6." in: Immunity, Vol. 34, Issue 6, pp. 932-46, 2011 (PubMed).

Baumjohann, Okada, Ansel: "Cutting Edge: Distinct waves of BCL6 expression during T follicular helper cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2089-92, 2011 (PubMed).

Bartlett, Slater, Glanville et al.: "Defining critical roles for NF-κB p65 and type I interferon in innate immunity to rhinovirus." in: EMBO molecular medicine, Vol. 4, Issue 12, pp. 1244-60, 2012 (PubMed).