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Mitofusin 2 (MFN2) (AA 661-757) antibody

Details for Product No. ABIN564263
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Antigen
Synonyms
CG3869, Dmel\\CG3869, MARF, Marf-1, Mfn, anon-WO0125274.3, dMFN, dMfn, dmfn, marf, mfn, mfn2, MFN2, hsg, cmt2a, cprp1, cmt2a2, CMT2A, CMT2A2, CPRP1, HSG, D630023P19Rik, Fzo, mg:cb01g09, si:dkeyp-104h9 ... show more
CG3869, Dmel\\CG3869, MARF, Marf-1, Mfn, anon-WO0125274.3, dMFN, dMfn, dmfn, marf, mfn, mfn2, MFN2, hsg, cmt2a, cprp1, cmt2a2, CMT2A, CMT2A2, CPRP1, HSG, D630023P19Rik, Fzo, mg:cb01g09, si:dkeyp-104h9.2, wu:fb79a11 show less
Epitope
AA 661-757
(43), (36), (11), (8), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(68), (67), (51), (14), (14), (14), (12), (2), (2), (1), (1), (1)
Host
Mouse
(54), (53), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA, RNA Interference (RNAi)
(93), (50), (36), (27), (17), (10), (4), (3), (2)
Pubmed 6 references available
Quantity 100 µg
Shipping to United States (Change)
Availability Will be delivered in 11 to 12 Business Days
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Catalog No. ABIN564263
394.24 $
Plus shipping costs $45.00

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Immunogen MFN2 (NP_055689, 661 a.a. ~ 757 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: FKRQFVEHASEKLQLVISYT GSNCSHQVQQELSGTFAHLC QQVDVTRENLEQEIAAMNKK IEVLDSLQSKAKLLRNKAGW LDSELNMFTHQYLQPSR
Clone 4H8
Isotype IgG2a kappa
Characteristics Mouse monoclonal antibody raised against a partial recombinant MFN2.
Alternative Name Mitofusin-2
Background Gene: mitofusin 2
Synonyms: CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF
Gene ID 9927
NCBI Accession NM_014874, NP_055689
Application Notes Application specialization: Cell lysate, Recombinant protein, Transfected lysate
Restrictions For Research Use only
Buffer 1x PBS, pH 7.2
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.
Supplier Images
anti-Mitofusin 2 (MFN2) (AA 661-757) antibody Immunoperoxidase of monoclonal antibody to MFN2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 5 µg/mL]
anti-Mitofusin 2 (MFN2) (AA 661-757) antibody (2) MFN2 monoclonal antibody (M03), clone 4H8 Western Blot analysis of MFN2 expression in HeLa (ABIN1339522).
anti-Mitofusin 2 (MFN2) (AA 661-757) antibody (3) Western blot analysis of MFN2 over-expressed 293 cell line, cotransfected with MFN2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MFN2 monoclonal antibody (M03), clone 4H8 (ABIN564263). GAPDH (36.1 kDa) used as specificity and loading control.
anti-Mitofusin 2 (MFN2) (AA 661-757) antibody (4) Detection limit for recombinant GST tagged MFN2 is approximately 1 ng/mL as a capture antibody.
anti-Mitofusin 2 (MFN2) (AA 661-757) antibody (5) Western Blot analysis of MFN2 expression in transfected 293T cell line by MFN2 monoclonal antibody (M03), clone 4H8.

Lane 1: MFN2 transfected lysate(86.4 kDa).
Lane 2: Non-transfected lysate.
anti-Mitofusin 2 (MFN2) (AA 661-757) antibody (6) MFN2 monoclonal antibody (M03), clone 4H8. Western Blot analysis of MFN2 expression in PC-12 (ABIN1339517).
anti-Mitofusin 2 (MFN2) (AA 661-757) antibody (7) Western Blot detection against Immunogen (36.41 KDa) .
Product cited in: Holloway, Perry, Thrush et al.: "PGC-1alpha's relationship with skeletal muscle palmitate oxidation is not present with obesity despite maintained PGC-1alpha and PGC-1beta protein." in: American journal of physiology. Endocrinology and metabolism, Vol. 294, Issue 6, pp. E1060-9, 2008 (PubMed).

Morán, Rivera, Sánchez-Aragó et al.: "Mitochondrial bioenergetics and dynamics interplay in complex I-deficient fibroblasts." in: Biochimica et biophysica acta, Vol. 1802, Issue 5, pp. 443-53, 2010 (PubMed).

Cosson, Marchetti, Ravazzola et al.: "Mitofusin-2 independent juxtaposition of endoplasmic reticulum and mitochondria: an ultrastructural study." in: PLoS ONE, Vol. 7, Issue 9, pp. e46293, 2012 (PubMed).

Lally, Herbst, Matravadia et al.: "Over-expressing mitofusin-2 in healthy mature mammalian skeletal muscle does not alter mitochondrial bioenergetics." in: PLoS ONE, Vol. 8, Issue 1, pp. e55660, 2013 (PubMed).

Russell, Lamon, Boon et al.: "Regulation of miRNAs in human skeletal muscle following acute endurance exercise and short-term endurance training." in: The Journal of physiology, Vol. 591, Issue Pt 18, pp. 4637-53, 2013 (PubMed).

Fiuza-Luces, Delmiro, Soares-Miranda et al.: "Exercise training can induce cardiac autophagy at end-stage chronic conditions: Insights from a graft-versus-host-disease mouse model." in: Brain, behavior, and immunity, 2013 (PubMed).

Validation Images
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