MFN2 antibody (AA 601-757)

Catalog No. ABIN3042362

Product Details anti-MFN2 Antibody

Target: MFN2 (Mitofusin 2 (MFN2))

Binding Specificity: AA 601-757

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFN2 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Mitofusin-2(MFN2) detection. Tested with WB in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Mitofusin-2(MFN2) detection. Tested with WB in Human.
Gene Name: mitofusin 2
Protein Name: Mitofusin-2

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Mitofusin 2 recombinant protein (Position: V601-R757). Human Mitofusin 2 shares 96% and 95% amino acid (aa) sequence identity with mouse and rat Mitofusin 2, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Mitofusin 2 is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-MFN2 antibody (ABIN3042362)

Montaigne, Marechal, Coisne, Debry, Modine, Fayad, Potelle, El Arid, Mouton, Sebti, Duez, Preau, Remy-Jouet, Zerimech, Koussa, Richard, Neviere, Edme, Lefebvre, Staels: "Myocardial contractile dysfunction is associated with impaired mitochondrial function and dynamics in type 2 diabetic but not in obese patients." in: Circulation, Vol. 130, Issue 7, pp. 554-64, (2014) (PubMed).

Target Details for MFN2

Target: MFN2 (Mitofusin 2 (MFN2))

Alternative Name: MFN2 (MFN2 Products)

Synonyms: CG3869 antibody, Dmel\\CG3869 antibody, MARF antibody, Marf-1 antibody, Mfn antibody, anon-WO0125274.3 antibody, dMFN antibody, dMfn antibody, dmfn antibody, marf antibody, mfn antibody, mfn2 antibody, MFN2 antibody, hsg antibody, cmt2a antibody, cprp1 antibody, cmt2a2 antibody, CMT2A antibody, CMT2A2 antibody, CPRP1 antibody, HSG antibody, D630023P19Rik antibody, Fzo antibody, mg:cb01g09 antibody, si:dkeyp-104h9.2 antibody, wu:fb79a11 antibody, mitofusin 2 antibody, Mitochondrial assembly regulatory factor antibody, mitofusin-2 antibody, mitofusin 2 L homeolog antibody, MFN2 antibody, Marf antibody, mfn2 antibody, LOC100186475 antibody, Mfn2 antibody, mfn2.L antibody

Background: Mitofusin-2 is a protein that in humans is encoded by the MFN2 gene. It is mapped to chromosome 1 and encodes a 757-amino acid protein that contains an ATP/GTP-binding site motif. This gene is expressed in many tissues and cell lines such as brain and KG-1 with the highest expression in heart and skeletal muscle. It has been found that MFN2 triggers mitochondrial energization, at least in part, by regulating OXPHOS expression through signals that are independent of its role as a mitochondrial fusion protein. And it contributes to the maintenance and operation of the mitochondrial network. Axonal CMT type 2A and autosomal dominant HMSN VI are caused by MFN2 and mutations in MFN2, which emphasizes its important role of mitochondrial function for both optic atrophies and peripheral neuropathies.

Synonyms: CMT2A antibody|CMT2A2 antibody|CPRP 1 antibody|CPRP1 antibody|Fzo antibody|HSG antibody|hyperplasia suppressor gene antibody|Hypertension related protein 1 antibody|KIAA0214 antibody|MARF antibody|MFN 2 antibody|Mfn2 antibody|MFN2_HUMAN antibody|Mitochondrial assembly regulatory factor antibody|Mitofusin-2 antibody|Mitofusin2 antibody|Transmembrane GTPase MFN2 antibody

Gene ID: 9927

UniProt: O95140

Pathways: Skeletal Muscle Fiber Development