NEDD1 antibody (AA 561-660)

Catalog No. ABIN566771

The Mouse Monoclonal anti-NEDD1 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect NEDD1 in samples from Human. There are 9+ publications available.

Quick Overview for NEDD1 antibody (AA 561-660) (ABIN566771)

Target: NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEDD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7D10

Product Details anti-NEDD1 Antibody

Binding Specificity: AA 561-660

Purpose: Mouse monoclonal antibody raised against a partial recombinant NEDD1.

Sequence: AGVASSLSEK IADSIGNNRQ NAPLTSIQIR FIQNMIQETL DDFREACHRD IVNLQVEMIK QFHMQLNEMH SLLERYSVNE GLVAEIERLR EENKRLRAHF

Cross-Reactivity: Human, Mouse

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: NEDD1 (NP_690869, 561 a.a. ~ 660 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-NEDD1 antibody (ABIN566771)

Uehara, Kamasaki, Hiruma, Poser, Yoda, Yajima, Gerlich, Goshima: "Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission." in: Molecular biology of the cell, Vol. 27, Issue 5, pp. 812-27, (2016) (PubMed).

Lecland, Lüders: "The dynamics of microtubule minus ends in the human mitotic spindle." in: Nature cell biology, Vol. 16, Issue 8, pp. 770-8, (2014) (PubMed).

Stiess, Maghelli, Kapitein, Gomis-Rüth, Wilsch-Bräuninger, Hoogenraad, Toli?-Nørrelykke, Bradke: "Axon extension occurs independently of centrosomal microtubule nucleation." in: Science (New York, N.Y.), Vol. 327, Issue 5966, pp. 704-7, (2010) (PubMed).

Archinti, Lacasa, Teixidó-Travesa, Lüders: "SPICE--a previously uncharacterized protein required for centriole duplication and mitotic chromosome congression." in: Journal of cell science, Vol. 123, Issue Pt 18, pp. 3039-46, (2010) (PubMed).

Uehara, Goshima: "Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase." in: The Journal of cell biology, Vol. 191, Issue 2, pp. 259-67, (2010) (PubMed).

Teixidó-Travesa, Villén, Lacasa, Bertran, Archinti, Gygi, Caelles, Roig, Lüders: "The gammaTuRC revisited: a comparative analysis of interphase and mitotic human gammaTuRC redefines the set of core components and identifies the novel subunit GCP8." in: Molecular biology of the cell, Vol. 21, Issue 22, pp. 3963-72, (2010) (PubMed).

Haren, Stearns, Lüders: "Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components." in: PLoS ONE, Vol. 4, Issue 6, pp. e5976, (2009) (PubMed).

Zhu, Fang, Fang: "FAM29A, a target of Plk1 regulation, controls the partitioning of NEDD1 between the mitotic spindle and the centrosomes." in: Journal of cell science, Vol. 122, Issue Pt 15, pp. 2750-9, (2009) (PubMed).

Zhu, Coppinger, Jang, Yates, Fang: "FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle." in: The Journal of cell biology, Vol. 183, Issue 5, pp. 835-48, (2008) (PubMed).

Target Details for NEDD1

Target: NEDD1 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 1 (NEDD1))

Alternative Name: NEDD1

Background: Full Gene Name: neural precursor cell expressed, developmentally down-regulated 1
Synonyms: FLJ35902,GCP-WD,TUBGCP7

Gene ID: 121441

NCBI Accession: NM_152905

Pathways: M Phase