ADH1 antibody (Biotin)

Catalog No. ABIN568065

The Biotin-conjugated Rabbit Polyclonal anti-ADH1 antibody (ABIN568065) specifically detects ADH1 in WB, IP, EIA, DB, ID, IF and RIA. The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for ADH1 antibody (Biotin) (ABIN568065)

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADH1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunofluorescence (IF), Radioimmunoassay (RIA)

Product Details anti-ADH1 Antibody (Biotin)

Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. Recognizes Alcohol Dehydrogenase.

Characteristics: Molar Ratio: ~5.5

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.

Immunogen: Alcohol dehydrogenase isolated and purified from Baker's Yeast. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water.

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for ADH1

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Alternative Name: Alcohol Dehydrogenase 1

Background: ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products. With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes. ADH play a major role in ethanol metabolism.Synonyms: ADC1, ADH1, Alcohol dehydrogenase I, O0947, YADH-1, YOL086C

Gene ID: 854068

NCBI Accession: NP_014555

UniProt: P00330