Glycerol Kinase antibody (Biotin)

Catalog No. ABIN568238

The Rabbit Polyclonal anti-Glycerol Kinase antibody is suitable to detect Glycerol Kinase in samples from Bacillus. It has been validated for WB, IF, IP, EIA, ID, RIA and DB.

Quick Overview for Glycerol Kinase antibody (Biotin) (ABIN568238)

Target: Glycerol Kinase (GK)

Reactivity: Bacillus

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glycerol Kinase antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Radioimmunoassay (RIA), Dot Blot (DB)

Product Details anti-Glycerol Kinase Antibody (Biotin)

Characteristics: Molar Ratio: Biotin/IgG ~4.6

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Glycerokinase isolated and purified from Bacillus stearothermophilus. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for Glycerol Kinase

Target: Glycerol Kinase (GK)

Alternative Name: Glycerol Kinase

Background: Glycerol kinase catalyzes the formation of glycerol 3 phosphate from ATP and glycerol. Dihydroxyacetone and L glyceraldehyde can also act as acceptors, UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway.Synonyms: ATP:glycerol 3-phosphotransferase, GK, GKD, Glycerokinase

Gene ID: 939741

NCBI Accession: NP_388810

UniProt: P18157