S1PR2 antibody (Internal Region)

Catalog No. ABIN568703

There is 1 publication for this product available. The Goat Polyclonal anti-S1PR2 antibody is suitable to detect S1PR2 in samples from Human, Mouse, Rat and Dog. It has been validated for WB and EIA.

Quick Overview for S1PR2 antibody (Internal Region) (ABIN568703)

Target: S1PR2 (Sphingosine-1-Phosphate Receptor 2 (S1PR2))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This S1PR2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-S1PR2 Antibody

Binding Specificity: Internal Region

Sequence: C-NYTKETLETQ

Specificity: Recognizes EDG-5 / S1P2.

Cross-Reactivity (Details): Species reactivity (expected):Dog.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_004221.3. Genename: S1PR2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-S1PR2 antibody (ABIN568703)

Song, Hou, Chen, Cheng, Lei, Cui, Sun, Wang, Fang: "Sphingosine-1-phosphate Receptor 2 Signaling Promotes Caspase-11-dependent Macrophage Pyroptosis and Worsens Escherichia coli Sepsis Outcome." in: Anesthesiology, Vol. 129, Issue 2, pp. 311-320, (2019) (PubMed).

Target Details for S1PR2

Target: S1PR2 (Sphingosine-1-Phosphate Receptor 2 (S1PR2))

Alternative Name: EDG-5 / S1P2

Background: The EDG (endothelial differentiation gene) family of G protein-coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high-affinity. EDG-6 is a low-affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain. EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.Synonyms: AGR16, EDG5, Endothelial differentiation G-protein coupled receptor 5, Gpcr13, H218, LPB2, S1P receptor 2, S1PR2, Sphingosine 1-phosphate receptor 2

Gene ID: 9294

NCBI Accession: NP_004221

UniProt: O95136

Pathways: Synaptic Membrane