SLCO1B3 antibody (Internal Region)

Catalog No. ABIN570800

The Goat Polyclonal anti-SLCO1B3 antibody is suitable to detect SLCO1B3 in samples from Human. It has been validated for ELISA, WB, IF and FACS.

Quick Overview for SLCO1B3 antibody (Internal Region) (ABIN570800)

Target: SLCO1B3 (Solute Carrier Organic Anion Transporter Family, Member 1B3 (SLCO1B3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SLCO1B3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-SLCO1B3 Antibody

Binding Specificity: Internal Region

Purpose: SLCO1B3

Sequence: NGTSPEIVEK D

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NGTSPEIVEKD, from the internal region of the protein sequence according to NP_062818.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in lysates of cell line HEK293 and A431 (calculated MW of 74.2 kDa according to NP_001336849.1 and 77.4 kDa acording to NP_062818.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperat

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SLCO1B3

Target: SLCO1B3 (Solute Carrier Organic Anion Transporter Family, Member 1B3 (SLCO1B3))

Alternative Name: SLCO1B3

Background: SLCO1B3, solute carrier organic anion transporter family, member 1B3, LST-3TM13, LST3, OATP1B3, OATP8, SLC21A8, liver-specific organic anion transporter 3TM13, organic anion transporter LST-3c, solute carrier family 21 (organic anion transporter), member

Gene ID: 28234, 28253

NCBI Accession: NP_062818, NP_001336849