NDUFS1 antibody (Internal Region)

Catalog No. ABIN571198

This Goat Polyclonal antibody specifically detects NDUFS1 in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for NDUFS1 antibody (Internal Region) (ABIN571198)

Target: NDUFS1 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase) (NDUFS1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NDUFS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-NDUFS1 Antibody

Binding Specificity: Internal Region

Purpose: NDUFS1

Sequence: TEKSATYVNT EGR

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TEKSATYVNTEGR, from the internal region of the protein sequence according to NP_004997.4.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 80 kDa band observed in Human Heart and Mouse Heart lysates (calculated MW of 79.5 kDa according to NP_004997.4). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NDUFS1

Target: NDUFS1 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 1, 75kDa (NADH-Coenzyme Q Reductase) (NDUFS1))

Alternative Name: NDUFS1

Background: NDUFS1, NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75 kDa (NADH-coenzyme Q reductase), CI-75Kd, MGC26839, PRO1304, NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75 kDa, NADH-coenzyme Q reductase, OTTHUMP00000206345, complex I, mitochondrial respirato

Gene ID: 4719, 227197, 301458

NCBI Accession: NP_004997