NDUFS2 antibody (Internal Region)

Catalog No. ABIN571199

The Goat Polyclonal anti-NDUFS2 antibody is suitable to detect NDUFS2 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for NDUFS2 antibody (Internal Region) (ABIN571199)

Target: NDUFS2 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2, 49kDa (NADH-Coenzyme Q Reductase) (NDUFS2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NDUFS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-NDUFS2 Antibody

Binding Specificity: Internal Region

Purpose: NDUFS2

Sequence: SRGVRQWQPD VE

Specificity: This antibody is expected to recognize both reported isoforms (NP_004541.1, NP_001159631.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SRGVRQWQPDVE, from the internal region of the protein sequence according to NP_004541.1, NP_001159631.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 51.9 kDa according to NP_001159631.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NDUFS2

Target: NDUFS2 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2, 49kDa (NADH-Coenzyme Q Reductase) (NDUFS2))

Alternative Name: NDUFS2

Background: NDUFS2, NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49 kDa (NADH-coenzyme Q reductase), NADH dehydrogenase (ubiquinone) Fe-S protein 2, NADH-ubiquinone oxidoreductase NDUFS2 subunit, complex 1, mitochondrial respiratory chain, 49-KD subunit

Gene ID: 4720, 226646, 289218

NCBI Accession: NP_004541, NP_001159631