SMARCA4 antibody (C-Term)

Catalog No. ABIN574431

The Mouse Monoclonal anti-SMARCA4 antibody (Clone BRG-01) (ABIN574431) specifically detects SMARCA4 in WB, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for SMARCA4 antibody (C-Term) (ABIN574431)

Target: SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMARCA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BRG-01

Product Details anti-SMARCA4 Antibody

Binding Specificity: C-Term

Specificity: The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205 kD catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.

Purification: Protein A purified

Immunogen: Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody,

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide,  pH 7.4. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for SMARCA4

Target: SMARCA4 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 4 (SMARCA4))

Alternative Name: SMARCA4 / BRG1

Background: Name/Gene ID: SMARCA4

Synonyms: SMARCA4, ATP-dependent helicase SMARCA4, BAF190, BRG1-associated factor 190A, Brahma protein-like 1, BRG1, BRM/SWI2-related gene 1, HSNF2b, MRD16, Protein BRG-1, SNF2-like 4, SNF2LB, SNF2-BETA, SNF2B, SWI2, Nuclear protein GRB1, SNF2, SNF2L4, Transcription activator BRG1, Homeotic gene regulator, Protein brahma homolog 1, RTPS2, Sucrose nonfermenting-like 4

Gene ID: 6597

UniProt: P51532

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance