Fibroblasts, Epithelial Cells antibody

Catalog No. ABIN601321

This Mouse Monoclonal antibody specifically detects Fibroblasts, Epithelial Cells in FACS, IP, IHC (fro) and IHC. It exhibits reactivity toward Human.

Quick Overview for Fibroblasts, Epithelial Cells antibody (ABIN601321)

Target: Fibroblasts, Epithelial Cells

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Product Details

Specificity: Recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.

Purification: Protein G purified

Immunogen: Human foreskin fibroblasts.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:50 - 1:200), IHC, IHC-Fr (1:100), IP

Usage: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Flow Cytometry: 1:50-1:200, 10 μL labels 10^6 cells in 100 μL. Immunohistochemistry (frozen): 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Target Details

Target: Fibroblasts, Epithelial Cells

Background: This antibody detects: Fibroblasts + Epithelial Cells