BCAT1 antibody (C-Term)

Catalog No. ABIN6746265

This anti-BCAT1 antibody is a Rabbit Polyclonal antibody detecting BCAT1 in WB. Suitable for Human, Mouse, Rat, Monkey and Guinea Pig. This Primary Antibody has been cited in 4+ publications.

Quick Overview for BCAT1 antibody (C-Term) (ABIN6746265)

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Reactivity: Human, Mouse, Rat, Monkey, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BCAT1 Antibody

Binding Specificity: C-Term

Specificity: Mouse BCAT1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Sheep, Dog, Bovine, Rabbit, Horse (92%) Mouse, Chicken (85%) Rat (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of mouse Bcat1 (Q8CBC8, NP_001019639). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Platypus (100%), Galago, Sheep, Dog, Bovine, Bat, Rabbit, Horse (92%), Opossum, Turkey, Chicken (85%), Rat (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-BCAT1 antibody (ABIN6746265)

Nakatani, Thompson, Barthel, Sakaue, Liu, Weigel, Roth: "Up-regulation of Akt3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21528-32, (1999) (PubMed).

Frech, Andjelkovic, Ingley, Reddy, Falck, Hemmings: "High affinity binding of inositol phosphates and phosphoinositides to the pleckstrin homology domain of RAC/protein kinase B and their influence on kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 13, pp. 8474-81, (1997) (PubMed).

Cross, Alessi, Cohen, Andjelkovich, Hemmings: "Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B." in: Nature, Vol. 378, Issue 6559, pp. 785-9, (1996) (PubMed).

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, (1991) (PubMed).

Target Details for BCAT1

Target: BCAT1 (Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1))

Alternative Name: BCAT1 / ECA39

Background: Name/Gene ID: BCAT1

Synonyms: BCAT1, BCAT(c), BCATC, ECA39, MECA39, BCT1, Placental protein 18, PNAS121, PP18, Protein ECA39

Gene ID: 586

NCBI Accession: NP_001019639

UniProt: P54687