SMPDL3A antibody (AA 82-131)

Catalog No. ABIN6746346

Product Details anti-SMPDL3A Antibody

Target: SMPDL3A (Sphingomyelin phosphodiesterase, Acid-Like 3A (SMPDL3A))

Binding Specificity: AA 82-131

Reactivity: Human, Mouse, Guinea Pig, Zebrafish (Danio rerio), Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPDL3A antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse SMPDL3A

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Guinea pig (100%) Dog, Rabbit, Horse, Pig (92%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa82-131 of mouse Smpdl3a (P70158, NP_065586). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Guinea pig (100%), Galago, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Turkey, Chicken, Platypus, Lizard (92%), Opossum, Zebra finch, Xenopus, Stickleback, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-SMPDL3A antibody (ABIN6746346)

Izawa, Nishizawa, Ohtakara, Inagaki: "Densin-180 interacts with delta-catenin/neural plakophilin-related armadillo repeat protein at synapses." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 5345-50, (2002) (PubMed).

Nürnberger, Bacallao, Phillips: "Inversin forms a complex with catenins and N-cadherin in polarized epithelial cells." in: Molecular biology of the cell, Vol. 13, Issue 9, pp. 3096-106, (2002) (PubMed).

Bhowmick, Ghiassi, Bakin, Aakre, Lundquist, Engel, Arteaga, Moses: "Transforming growth factor-beta1 mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism." in: Molecular biology of the cell, Vol. 12, Issue 1, pp. 27-36, (2001) (PubMed).

Miyatani, Shimamura, Hatta, Nagafuchi, Nose, Matsunaga, Hatta, Takeichi: "Neural cadherin: role in selective cell-cell adhesion." in: Science (New York, N.Y.), Vol. 245, Issue 4918, pp. 631-5, (1989) (PubMed).

Target Details for SMPDL3A

Target: SMPDL3A (Sphingomyelin phosphodiesterase, Acid-Like 3A (SMPDL3A))

Alternative Name: SMPDL3A (SMPDL3A Products)

Background: Name/Gene ID: SMPDL3A
Family: Phosphodiesterase - Other

Synonyms: SMPDL3A, ASM-like phosphodiesterase 3a, ASML3a, YR36GH4.1, ASM3A

Gene ID: 10924

NCBI Accession: NP_065586

UniProt: Q92484