DNAJC2 antibody (AA 417-466)

Catalog No. ABIN6746696

Cited in 3+ publications. The Rabbit Polyclonal anti-DNAJC2 antibody (ABIN6746696) specifically detects DNAJC2 in WB. The antibody is reactive with Human samples.

Quick Overview for DNAJC2 antibody (AA 417-466) (ABIN6746696)

Target: DNAJC2 (DnaJ (Hsp40) Homolog, Subfamily C, Member 2 (DNAJC2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJC2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DNAJC2 Antibody

Binding Specificity: AA 417-466

Specificity: Human DNAJC2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Rabbit, Guinea pig (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa417-466 of human DNAJC2 (Q99543, NP_055192). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset (100%), Monkey, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig, Platypus (92%), Galago (86%), Bovine, Bat, Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-DNAJC2 antibody (ABIN6746696)

Fallon, Moreau, Croft, Labib, Gu, Fon: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, (2002) (PubMed).

Zong, Ren, Young, Pypaert, Mu, Birnbaum, Shulman: "AMP kinase is required for mitochondrial biogenesis in skeletal muscle in response to chronic energy deprivation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 15983-7, (2002) (PubMed).

Brocke, Chiang, Wagner, Schulman: "Functional implications of the subunit composition of neuronal CaM kinase II." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22713-22, (1999) (PubMed).

Target Details for DNAJC2

Target: DNAJC2 (DnaJ (Hsp40) Homolog, Subfamily C, Member 2 (DNAJC2))

Alternative Name: DNAJC2 / ZRF1

Background: Name/Gene ID: DNAJC2

Synonyms: DNAJC2, MPHOSPH11, Zuotin-related factor 1, ZUO1, ZRF1, M-phase phosphoprotein 11, MPP11, Zuotin, Zuotin related factor 1

Gene ID: 27000

NCBI Accession: NP_055192

UniProt: Q99543

Pathways: Chromatin Binding