ADRA1D antibody (AA 286-335)

Catalog No. ABIN6746725

This Rabbit Polyclonal antibody specifically detects ADRA1D in WB. It exhibits reactivity toward Human, Rat, Mouse, Dog, Horse, Cow, Guinea Pig, Rabbit, Monkey, Pig and Bat and has been mentioned in 3+ publications.

Quick Overview for ADRA1D antibody (AA 286-335) (ABIN6746725)

Target: ADRA1D (Adrenergic Receptor, alpha 1d (ADRA1D))

Reactivity: Human, Rat, Mouse, Dog, Horse, Cow, Guinea Pig, Rabbit, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRA1D antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ADRA1D Antibody

Binding Specificity: AA 286-335

Specificity: Human ADRA1D

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Rabbit, Pig, Guinea pig (100%) Sheep (92%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa286-335 of human ADRA1D (P25100, NP_000669). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Marmoset, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Sheep (92%), Mouse, Rat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-ADRA1D antibody (ABIN6746725)

Mirski, Gerlach, Cole: "Sequence determinants of nuclear localization in the alpha and beta isoforms of human topoisomerase II." in: Experimental cell research, Vol. 251, Issue 2, pp. 329-39, (1999) (PubMed).

Brown, Holden, Rahn, Perkins: "Immunohistochemical staining for DNA topoisomerase IIa in Hodgkin's disease." in: American journal of clinical pathology, Vol. 109, Issue 1, pp. 39-44, (1998) (PubMed).

Herzog, Holmes, Tuschong, Ganapathi, Zwelling: "Absence of topoisomerase IIbeta in an amsacrine-resistant human leukemia cell line with mutant topoisomerase IIalpha." in: Cancer research, Vol. 58, Issue 23, pp. 5298-300, (1998) (PubMed).

Target Details for ADRA1D

Target: ADRA1D (Adrenergic Receptor, alpha 1d (ADRA1D))

Alternative Name: ADRA1D

Background: Name/Gene ID: ADRA1D
Subfamily: Adrenergic
Family: GPCR

Synonyms: ADRA1D, Alpha 1d, Alpha-1D adrenergic receptor, ADRA1R, Alpha-1D adrenoceptor, Alpha-1D adrenoreceptor, ALPHA1, Alpha-1D-adrenergic receptor, Alpha-adrenergic receptor 1a, Alpha1delta-AR, DAR, ADRA1, Adrenoceptor alpha 1D, Alpha-1A adrenergic receptor, DJ779E11.2

Gene ID: 146

NCBI Accession: NP_000669

UniProt: P25100