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Donkey anti-Sheep (Ovine) IgG (Heavy & Light Chain) Antibody
Heavy & Light Chain
Flow Cytometry (FACS), Immunofluorescence (IF)
|3 references available|
|Quantity||1 mg (1 mg/ml (E 1% at 280 nm = 13.0)) (Variants)|
|Price||180.71 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||Purified Sheep IgG, whole molecule|
|Description||Donkey anti-Sheep IgG (H&L) - Affinity Pure, DyLight 633 Conjugate, min x w/human,mouse, or rabbit IgG. Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian. Fluorphore: DyLight 633 (Ex = 638 nm, Em = 658 nm). Fluor Protein Ratio: Moles DyLight 633 per Mole Antibody.|
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
|Application Notes||This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications|
|Concentration||1 mg/ml (E 1% at 280 nm = 13.0)|
|Purity||Affinity purified antibody is > 95% based on SDS-PAGE|
|Purification||Affinity purified using solid phase bovine IgG (H&L)|
|Buffer||10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide|
|Storage||Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.|
|Research Area||Secondary Antibodies|
|Restrictions||For Research Use only|
Wang, Yin, Chao et al.: "BID: a novel BH3 domain-only death agonist." in: Genes & development, Vol. 10, Issue 22, pp. 2859-69, 1996 (PubMed).
Kudla, Montessuit, Eskes et al.: "The destabilization of lipid membranes induced by the C-terminal fragment of caspase 8-cleaved bid is inhibited by the N-terminal fragment." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 22713-8, 2000 (PubMed).
Zhuang, Demirs, Kochevar: "p38 mitogen-activated protein kinase mediates bid cleavage, mitochondrial dysfunction, and caspase-3 activation during apoptosis induced by singlet oxygen but not by hydrogen peroxide." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 25939-48, 2000 (PubMed).