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Donkey anti-Sheep (Ovine) IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN611866, Supplier: Login to see
Antigen
Epitope
Heavy & Light Chain
4274
1867
1736
964
643
277
203
57
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Sheep (Ovine)
2414
2330
2036
1524
1260
608
562
383
328
296
295
291
290
224
162
112
105
76
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
Host
Donkey
6022
4118
1526
608
545
262
85
49
43
8
8
4
4
3
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
Supplier
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Immunogen Purified Sheep IgG, whole molecule
Characteristics Donkey anti-Sheep IgG (H&L) - Affinity Pure, DyLight 633 Conjugate.
Fluorphore: DyLight 633 (Ex = 638 nm, Em = 658 nm).
Fluor Protein Ratio: Moles DyLight 633 per Mole Antibody.
Purification Affinity purified using solid phase bovine IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C
Product cited in: Zhuang, Demirs, Kochevar: "p38 mitogen-activated protein kinase mediates bid cleavage, mitochondrial dysfunction, and caspase-3 activation during apoptosis induced by singlet oxygen but not by hydrogen peroxide." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 25939-48, 2000 (PubMed).

Kudla, Montessuit, Eskes et al.: "The destabilization of lipid membranes induced by the C-terminal fragment of caspase 8-cleaved bid is inhibited by the N-terminal fragment." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 22713-8, 2000 (PubMed).

Background publications Wang, Yin, Chao et al.: "BID: a novel BH3 domain-only death agonist." in: Genes & development, Vol. 10, Issue 22, pp. 2859-69, 1996 (PubMed).