Goat anti-Mouse (Murine) IgG (Heavy & Light Chain) Antibody (HRP)

Antigen: IgG

Binding Site: Heavy & Light Chain

Host: Goat

Reactivity: Mouse (Murine)

Conjugate: HRP

Application: Immunoassay (IA)

Catalog no.: ABIN612280

Additional Information

Fragment: F(ab')2 fragment

Immunogen: Purified Mouse IgG, whole molecule

Format: Lyophilized

Description: Goat anti-Mouse IgG (H&L) - F(ab)'2 fragment, HRP Conjugate, min x w/bovine, goat, human, rabbit, or rat IgG (highly absorbed against rat IgG). Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

Specificity: 0.1 % (v/v) Kathon CG. Store freeze dried powder at 2-8 C.

Application Details

Application Notes: This conjugate is suitable for all all immunoassays applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5,000 for Immunohistochemistry, 1:200-1:5000 for ELISA/Western blot

Concentration: 1.5 mg/ml (E 1% at 280 nm = 13.0)

Purity: Affinity purified antibody is > 95% based on SDS-PAGE

Purification: Affinity purified using solid phase mouse IgG (H&L)

Buffer: Lyophilized. Affinity purified using solid phase cat IgG (H&L)

Storage: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free . Shelf Life: Store lyophilized material at 2-8 C. For long term storage after reconstitution, dilute with 50% glycerol, store at -20 C as a liquid. Prepare working dilution prior to use, then discard.

Research Area: Secondary Antibodies

Restrictions: For Research Use only

Publications

Product: Han, Lee, Bibbs et al.: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).

Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." in: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, 1996 (PubMed).

Winston, Chan, Johnson et al.: "Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, 1997 (PubMed).