HNF4A antibody (N-Term)

Catalog No. ABIN614896

This Goat Polyclonal antibody specifically detects HNF4A in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Pig and Dog.

Quick Overview for HNF4A antibody (N-Term) (ABIN614896)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reactivity: Human, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This HNF4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-HNF4A Antibody

Binding Specificity: N-Term

Sequence: RLSKTLVDMD MADY-C

Specificity: This antibody detects HNF4 alpha / TCF14 (N-term).

Cross-Reactivity (Details): Species reactivity (expected):Dog, PigSpecies reactivity (tested):Human

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide from (N-term) of human HNF4A Genename: HNF4A

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Tris saline buffer, pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4 alpha / TCF14

Background: Hepatocyte nuclear factor 4 alpha (HNF4 alpha), a NR2 Hepatocyte NF4-Like receptor, is a liver-enriched transcription factor that is involved in the expression of liver-specific genes. HNF4 alpha controls the expression of transcription factor HNF1, which is important in the expression of hepatic genes, including those involved in glucose, cholesterol, and fatty acid metabolism. HNF4 alpha is essential for hepatocyte differentiation during liver development, metabolic regulation, and liver function. Although HNF4 alpha is a tissue-specific transcription factor, it is also an essential regulator of early embryonic events. Mutations in the HNF4 alpha gene are responsible for type-1 maturity-onset diabetes of the young (MODY1), which is characterized by a defect in insulin secretion. Mutations in HNF4 alpha binding sites have been linked to hemophilia B Leyden, atherosclerosis, hepatitis, and other diseases. HNF4 alpha has been linked to liver cancer via hepatitis and hepatitis B viral infections. Mouse embryos lacking HNF4 alpha fail to complete gastrulation due to dysfunction of the visceral endoderm. Recently, it has been shown that HNF4 alpha is critically involved in the PXR- and CAR-mediated transcriptional activation of CYP3A4, a gene involved in the metabolism of a wide range of drugs Tirona et al. 2003. At least four isoforms (HNF4 alpha1/HNF4B, HNF4 alpha2/HNF4A, HNF4 alpha3/HNF4C, and HNF4 alpha4) are produced by alternative splicing.Synonyms: HNF4, HNF4A, Hepatocyte nuclear factor 4-alpha, NR2A1, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, Transcription factor HNF-4

Gene ID: 3172

NCBI Accession: NP_000448

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process