Mesothelin antibody (Internal Region)

Catalog No. ABIN615931

This Goat Polyclonal antibody specifically detects Mesothelin in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Mesothelin antibody (Internal Region) (ABIN615931)

Target: Mesothelin (MSLN)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Mesothelin Antibody

Binding Specificity: Internal Region

Sequence: CPSGKKAREI DES

Specificity: This antibody is expected to recognize isoform 1 and 2 (NP_005814.2, NP_037536.2) of Mesothelin (MSLN).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_005814.2, NP_037536.2. Genename: MSLN

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: Mesothelin

Background: Mesothelin is a glycosyl-phosphatidyl- inositol-anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71- kDa precursor protein that is processed to a 40- kDa glycosylphosphatidyl-inositol-anchored protein that composes the mature portion and an NH2-terminal 31- kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.Synonyms: CAK1 antigen, MPF, MSLN, Pre-pro-megakaryocyte-potentiating factor

Molecular Weight: 68.1kDa (NP_005814.2) and 69.0kDa (NP_037536.2).

Gene ID: 10232

NCBI Accession: NP_001170826

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process