GRP78 antibody

Catalog No. ABIN618937

This Goat Polyclonal antibody specifically detects GRP78 in WB. It exhibits reactivity toward Arabidopsis thaliana, Zea mays, Spinach and Barley.

Quick Overview for GRP78 antibody (ABIN618937)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Arabidopsis thaliana, Zea mays, Spinach, Barley

Host: Goat

Clonality: Polyclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRP78 Antibody

Purpose: Anti-BiP | Lumenal-binding protein (goat antibody)

Cross-Reactivity (Details): Arabidopsis thaliana, Hordeum vulgare, Spinacia oleracea, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Hordeum vulgare,Nicotiana tabacum,Oryza sativa,Picea sitchensis,Populus trichocarpa,Physcomitrium patens,Spinacia oleracea,Zea mays

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 UniProt:Q9LKR3

Application Details

Application Notes: 1 : 2000 (WB)

Comment:

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Antibody has a reduced reactivity to monocots in western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: BiP

Background: BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2

Molecular Weight: 73.5 | 80 kDa

Gene ID: 834207

UniProt: Q39043

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling