ALAD antibody (Middle Region)

Catalog No. ABIN632019

The Rabbit Polyclonal anti-ALAD antibody (ABIN632019) specifically detects ALAD in WB. The antibody is reactive with Human samples.

Quick Overview for ALAD antibody (Middle Region) (ABIN632019)

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ALAD Antibody

Binding Specificity: Middle Region

Specificity: ALAD antibody was raised against the middle region of ALAD

Purification: Affinity purified

Immunogen: ALAD antibody was raised using the middle region of ALAD corresponding to a region with amino acids SVMSYSAKFASCFYGPFRDAAKSSPAFGDRRCYQLPPGARGLALRAVDRD

Application Details

Application Notes: WB: 0.25 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

ALAD Blocking Peptide, (ABIN5612002), is also available for use as a blocking control in assays to test for specificity of this ALAD antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of ALAD antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for ALAD

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Alternative Name: ALAD

Background: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.

Molecular Weight: 39 kDa (MW of target protein)