MCM10 antibody (Middle Region)

Catalog No. ABIN634085

The Rabbit Polyclonal anti-MCM10 antibody is suitable to detect MCM10 in samples from Human. It has been validated for WB.

Quick Overview for MCM10 antibody (Middle Region) (ABIN634085)

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCM10 Antibody

Binding Specificity: Middle Region

Specificity: MCM10 antibody was raised against the middle region of MCM10

Purification: Affinity purified

Immunogen: MCM10 antibody was raised using the middle region of MCM10 corresponding to a region with amino acids PKLSALAEAKKLAAITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERV

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

MCM10 Blocking Peptide, (ABIN5614695), is also available for use as a blocking control in assays to test for specificity of this MCM10 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of MCM10 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for MCM10

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Alternative Name: MCM10

Background: This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding.

Molecular Weight: 98 kDa (MW of target protein)

Pathways: Mitotic G1-G1/S Phases, DNA Replication