MCM10 antibody (Middle Region)

Catalog No. ABIN2774890

The Rabbit Polyclonal anti-MCM10 antibody is suitable to detect MCM10 in samples from Human, Dog, Cow, Rabbit, Horse and Pig. It has been validated for WB.

Quick Overview for MCM10 antibody (Middle Region) (ABIN2774890)

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Reactivity: Human, Dog, Cow, Rabbit, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCM10 Antibody

Binding Specificity: Middle Region

Purpose: MCM10 Antibody - middle region

Sequence: PKLSALAEAK KLAAITKLRA KGQVLTKTNP NSIKKKQKDP QDILEVKERV

Predicted Reactivity: Cow: 77%, Dog: 86%, Horse: 93%, Human: 100%, Pig: 77%, Rabbit: 86%

Characteristics: This is a rabbit polyclonal antibody against MCM10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MCM10

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP36656-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for MCM10

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Alternative Name: MCM10

Background:

Background Information: This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Gene Name: Minichromosome maintenance complex component 10

Alternative Symbols: CNA43, DNA43, PRO2249

Protein Name: Protein MCM10 homolog

Molecular Weight: 98kDa

Gene ID: 55388

NCBI Accession: NP_060988

UniProt: B7ZKZ8

Pathways: Mitotic G1-G1/S Phases, DNA Replication