GLUT4 antibody

Catalog No. ABIN636172

This Rabbit Polyclonal antibody specifically detects GLUT4 in WB, IHC and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for GLUT4 antibody (ABIN636172)

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-GLUT4 Antibody

Cross-Reactivity (Details): This mouse GT4 peptide sequence is 100 % conserved in rat, human, bovine, and pig Glut-4, 91 % in bovine. It has no significant sequence homology with other gluts.

Purification: Affinity chromatography purified

Immunogen: GLUT4 antibody was raised in rabbit using a 12 amino acid peptide from mouse Glut-4 as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 1:10,000, HC: 2-10 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

A synthetic GLUT4 Blocking peptide is available for validating specificity of this GLUT4 antibody, (ABIN935385)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in PBS to a concentration of 1 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 0.1 % BSA.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Alternative Name: GLUT4

Background: Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-4 (mouse/human 509-aa) is primarily expressed in muscle and adipose tissue and regulated by insulin.

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effect