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LAMP1 antibody (Lysosomal-Associated Membrane Protein 1) (PE)

Details for Product anti-LAMP1 Antibody No. ABIN638446, Supplier: Login to see
Antigen
  • AI196048
  • CD107a
  • Lamp-1
  • LAMPA
  • LGP120
  • LEP100
Reactivity
Human, Non-Human Primate, Mouse (Murine)
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122
83
24
14
8
5
2
2
Host
Mouse
192
107
57
2
Clonality (Clone)
Monoclonal ()
Conjugate
This LAMP1 antibody is conjugated to PE
42
31
28
16
7
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4
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2
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2
2
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2
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2
2
2
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2
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2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
180
169
75
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44
33
26
19
14
12
4
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3
2
2
1
1
1
1
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1
Supplier
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Immunogen Human PBMC
Clone H4A3
Isotype IgG1
Specificity The mouse monoclonal antibody H4A3 recognizes CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Alternative Name LAMP1 (LAMP1 Antibody Abstract)
Background CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2 % of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Gene ID 3916
Research Area Cancer, Hematopoietic Progenitors, Organelles
Pathways
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 4 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 0.02 % sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Flow Cytometry (FACS) image for anti-LAMP1 antibody (Lysosomal-Associated Membrane Protein 1)  (PE) (ABIN638446) Surface staining of human peripheral blood cells with anti-CD107a (H4A3) PE.
Product cited in: Majer, Vlaskova, Krol et al.: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, 2012 (PubMed).

Mao, Tu, Liu et al.: "Inhibition of human natural killer cell activity by influenza virions and hemagglutinin." in: Journal of virology, Vol. 84, Issue 9, pp. 4148-57, 2010 (PubMed).

Yu, Gallegos, Marches et al.: "Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines." in: Blood, Vol. 112, Issue 9, pp. 3671-8, 2008 (PubMed).

Tomescu, Chehimi, Maino et al.: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, 2007 (PubMed).

Carlsten, Björkström, Norell et al.: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, 2007 (PubMed).

Deetz, Hebbeler, Propp et al.: "Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys." in: Infection and immunity, Vol. 74, Issue 8, pp. 4505-11, 2006 (PubMed).

Marcenaro, Gallo, Martini et al.: "Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease." in: Blood, Vol. 108, Issue 7, pp. 2316-23, 2006 (PubMed).

Furuta, Ikeda, Nakayama et al.: "Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases." in: The American journal of pathology, Vol. 159, Issue 2, pp. 449-55, 2001 (PubMed).

Mane, Marzella, Bainton et al.: "Purification and characterization of human lysosomal membrane glycoproteins." in: Archives of biochemistry and biophysics, Vol. 268, Issue 1, pp. 360-78, 1989 (PubMed).