Quick Overview for Phosphotyrosine antibody (ABIN640608)
Target
Phosphotyrosine
Host
Mouse
Clonality
Monoclonal
Conjugate
This Phosphotyrosine antibody is un-conjugated
Application
Western Blotting (WB), Immunofluorescence (IF), ELISA
Purpose
Mouse Anti-pTyr
Specificity
DS-MB-02120 recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the Tcell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets.
ELISA (dilution: , 2 μg/mL), Immunofluorescence, Western Blotting
Restrictions
For Research Use only
Reconstitution
Supplied as 500 µg of purified antibody. It is reconstituted with 1000 µl of sterile 100mM Borate buffered saline pH8.0. Its final concentration is 0.5 mg/ml. Please avoid freeze-thaw cycles.