Complement C4d antibody

Catalog No. ABIN640791

The Mouse Monoclonal anti-Complement C4d antibody (ABIN640791) specifically detects Complement C4d in ELISA, IF, WB, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for Complement C4d antibody (ABIN640791)

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement C4d antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Complement C4d Antibody

Purpose: Mouse Anti-Human C4d

Specificity: DS-MB-00163 recognizes the secreted protein complement component 4d (C4d). The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b's alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4d's short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. C4 has been linked to susceptibility to systemic lupus erythematosous. ImmunohistologyIt has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. DS-MB-00163 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 minutes at room temperature is beneficial (6).

Characteristics: Blood Coagulation Proteins

Purification: Purified

Immunogen: Native, from human plasma

Isotype: IgG1

Application Details

Application Notes: Immunohistology - Frozen, ELISA (dilution: 1/250, 1/600), Immunofluorescence, Immunohistology - Paraffin, Western Blotting

Restrictions: For Research Use only

Handling

Concentration: IgG concentration 1.0 mg/ml

Buffer: Borate buffered saline pH 8.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for Complement C4d

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Alternative Name: C4d