ATG5 antibody

Catalog No. ABIN650657

There is 1 publication for this product available. The Rabbit Polyclonal anti-ATG5 antibody is suitable to detect ATG5 in samples from Human. It has been validated for WB, IF and IHC (p).

Quick Overview for ATG5 antibody (ABIN650657)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB12327

Product Details anti-ATG5 Antibody

Purification: This antibody is purified through a protein A column, followed by dialysis against PBS.

Immunogen: This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length of human ATG5.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:200. WB: 1:1000. WB: 1:500. WB: 1:500. IHC-P: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ATG5 antibody (ABIN650657)

Jaber, Dou, Chen, Catanzaro, Jiang, Ballou, Selinger, Ouyang, Lin, Zhang, Zong: "Class III PI3K Vps34 plays an essential role in autophagy and in heart and liver function." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 6, pp. 2003-8, (2012) (PubMed).

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: APG5

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Molecular Weight: 32447

Gene ID: 9474

NCBI Accession: NP_004840

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy