Cofilin 1 (Non-Muscle) (CFL1) (Center) antibody

Antigen: Cofilin 1 (Non-Muscle) (CFL1)

Synonyms: CFL, xac1, MGC54000, cfl, MGC76274, cb86, sb:cb86, zgc:56501, wu:fb18a04, wu:fk77b03, CFL1, 12/8, ADF/cofilin, Adf, Cadf, D-cof, D61, T8, Tsr, cofilin, cofilin/ADF, cofilin/actin depolymerizing factor homolog, l(2)03101, l(2)k03101, l(2)k05633, l(2)k13409, ntf, DmelCG4254, CG4254, COFILIN

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN650759

Additional Information

Alternative name: CFL1

Gene ID: 1072

UniProt: P23528

Immunogen: This CFL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77~106 amino acids from the Center region of human CFL1.

Isotype: Ig

Description: Other names: Cofilin-1, Cofilin, non-muscle isoform, 18 kDa phosphoprotein, p18, CFL, CFL1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CFL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77~106 amino acids from the Center region of human CFL1.

Molecular Weight: 18502 DA

Comments:

Background: Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.

Synonyms: CFL, xac1, MGC54000, cfl, MGC76274, cb86, sb:cb86, zgc:56501, wu:fb18a04, wu:fk77b03, CFL1, 12/8, ADF/cofilin, Adf, Cadf, D-cof, D61, T8, Tsr, cofilin, cofilin/ADF, cofilin/actin depolymerizing factor homolog, l(2)03101, l(2)k03101, l(2)k05633, l(2)k13409, ntf, DmelCG4254, CG4254, COFILIN

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CFL1 Antibody (Center) (ABIN650759) in HL-60 cell line lysates (35ug/lane). CFL1 (arrow) was detected using the purified Pab.

Western blot analysis of CFL1 Antibody (Center) (ABIN650759) in mouse NIH-3T3 cell line lysates (35ug/lane). CFL1 (arrow) was detected using the purified Pab.

CFL1 Antibody (Center) (ABIN650759) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Fazal, Bijli, Minhajuddin et al.: "Essential role of cofilin-1 in regulating thrombin-induced RelA/p65 nuclear translocation and intercellular adhesion molecule 1 (ICAM-1) expression in endothelial cells." in: The Journal of biological chemistry, Vol. 284, Issue 31, pp. 21047-56, 2009 (PubMed).