Thioredoxin Domain Containing 12 (Endoplasmic Reticulum) (TXNDC12) (C-Term) antibody

Antigen: Thioredoxin Domain Containing 12 (Endoplasmic Reticulum) (TXNDC12)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN651065

Additional Information

Alternative name: TXNDC12

Gene ID: 51060

UniProt: O95881

Immunogen: This TXNDC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 140-170 from the C-terminal region of human TXNDC12.

Isotype: Ig

Description: Other names: hTLP19, Endoplasmic reticulum resident protein 18, ER protein 18, ERp18, Endoplasmic reticulum resident protein 19, ER protein 19, ERp19, Thioredoxin-like protein p19, Thioredoxin domain-containing protein 12,UNQ713/PRO1376, TLP19, TXNDC12

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TXNDC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 140-170 from the C-terminal region of human TXNDC12.

Molecular Weight: 19206 DA

Comments:

Background: TXNDC12 belongs to the thioredoxin superfamily (see TXN, MIM 187700). Members of this superfamily possess a thioredoxin fold with a consensus active-site sequence (CxxC) and have roles in redox regulation, defense against oxidative stress, refolding of disulfide-containing proteins, and regulation of transcription factors

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Protein Modifications, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of TXNDC12 Antibody (C-term) (ABIN651065) in Y79 cell line lysates (35ug/lane). TXNDC12 (arrow) was detected using the purified Pab.

Western blot analysis of TXNDC12 Antibody (C-term) (ABIN651065) in mouse stomach tissue lysates (35ug/lane). TXNDC12 (arrow) was detected using the purified Pab.

TXNDC12 Antibody (C-term) (ABIN651065) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TXNDC12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Jeong, Lee, Park et al.: "ERp16, an endoplasmic reticulum-resident thiol-disulfide oxidoreductase: biochemical properties and role in apoptosis induced by endoplasmic reticulum stress." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25557-66, 2008 (PubMed).