Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (N-Term) antibody

Antigen: Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN651212

Additional Information

Alternative name: CHRNA3

Gene ID: 1136

UniProt: P32297

Immunogen: This CHRNA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human CHRNA3.

Isotype: Ig

Description: Other names: Neuronal acetylcholine receptor subunit alpha-3,NACHRA3, CHRNA3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CHRNA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human CHRNA3.

Molecular Weight: 57480 DA

Comments:

Background: CHRNA3 locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. BS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Protein Modifications, Cancer, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of CHRNA3 Antibody (N-term) (ABIN651212) in CEM cell line lysates (35ug/lane).CHRNA3 (arrow) was detected using the purified Pab.

CHRNA3 Antibody (N-term) (ABIN651212) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHRNA3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Girard, Lou, Azzoli et al.: "Analysis of genetic variants in never-smokers with lung cancer facilitated by an Internet-based blood collection protocol: a preliminary report." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 2, pp. 755-63, 2010 (PubMed).