Zinc Finger, FYVE Domain Containing 16 (ZFYVE16) (N-Term) antibody

Antigen: Zinc Finger, FYVE Domain Containing 16 (ZFYVE16)

Synonyms: ENDOFIN, KIAA0305, DKFZp686E13162, AI035632, mKIAA0305, B130031L15, B130024H06Rik, ZFYVE16, RGD1564784

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN651313

Additional Information

Alternative name: ZFYVE16

Gene ID: 9765

UniProt: Q7Z3T8

Immunogen: This ZFYVE16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-410 amino acids from the N-terminal region of human ZFYVE16.

Isotype: Ig

Description: Other names: Endosome-associated FYVE domain protein, Endofin, Zinc finger FYVE domain-containing protein 16,KIAA0305, ZFYVE16

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ZFYVE16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-410 amino acids from the N-terminal region of human ZFYVE16.

Molecular Weight: 168849 DA

Comments:

Background: The ZFYVE16 gene encodes endofin, an endosomal protein implicated in regulating membrane trafficking. It is characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain positioned in the middle of the molecule.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies

Restrictions: For Research Use only

Images

Western blot analysis of ZFYVE16 Antibody (N-term) (ABIN651313) in K562 cell line lysates (35ug/lane).ZFYVE16 (arrow) was detected using the purified Pab.

ZFYVE16 Antibody (N-term) (ABIN651313) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZFYVE16 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ZFYVE16 Antibody (N-term) (ABIN651313) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Shi, Chang, Nie et al.: "Endofin acts as a Smad anchor for receptor activation in BMP signaling." in: Journal of cell science, Vol. 120, Issue Pt 7, pp. 1216-24, 2007 (PubMed).

Chen, Low, Choong et al.: "Phosphoproteomics identified Endofin, DCBLD2, and KIAA0582 as novel tyrosine phosphorylation targets of EGF signaling and Iressa in human cancer cells." in: Proteomics, Vol. 7, Issue 14, pp. 2384-97, 2007 (PubMed).