HIST1H1B (N-term) antibody

Antigen: HIST1H1B (N-term)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651424

Additional Information

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 3009

Swiss-Prot: P16401

Immunogen: This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the N-terminal region of human HIST1H1B.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Histone H1a, Histone H1.5,H1F5, HIST1H1B

Molecular Weight: 22580 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer

Restrictions: For Research Use only

Images

Western blot analysis of HIST1H1B Antibody (N-term) (ABIN651424) in NCI-H460 cell line lysates (35ug/lane).HIST1H1B (arrow) was detected using the purified Pab.

HIST1H1B Antibody (N-term) (ABIN651424) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.