Histone H1.5 (N-Term) antibody

Antigen: Histone H1.5

Synonyms: H1, H1B, H1.5, H1F5, MGC126630, MGC126632, H1f5, H1s-3

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651424

Additional Information

Alternative name: HIST1H1B

Gene ID: 3009

UniProt: P16401

Immunogen: This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the N-terminal region of human HIST1H1B.

Isotype: Ig

Description: Other names: Histone H1a, Histone H1.5,H1F5, HIST1H1B

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HIST1H1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-71 amino acids from the N-terminal region of human HIST1H1B.

Molecular Weight: 22580 DA

Comments:

Background: Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer

Restrictions: For Research Use only

Images

Western blot analysis of HIST1H1B Antibody (N-term) (ABIN651424) in NCI-H460 cell line lysates (35ug/lane).HIST1H1B (arrow) was detected using the purified Pab.

HIST1H1B Antibody (N-term) (ABIN651424) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Trojer, Li, Sims et al.: "L3MBTL1, a histone-methylation-dependent chromatin lock." in: Cell, Vol. 129, Issue 5, pp. 915-28, 2007 (PubMed).

Happel, Stoldt, Schmidt et al.: "M phase-specific phosphorylation of histone H1.5 at threonine 10 by GSK-3." in: Journal of molecular biology, Vol. 386, Issue 2, pp. 339-50, 2009 (PubMed).